Molecular Typing of Avian Pasteurella multocida lsolatesby PCR-RFLPof ompH Gene

Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragmentlength polymorphism (RFLP) of a species-specific PCR assay. Amplification was based on the gene ompH, encoding a major outer memberane protein. RFLP analysis of the 1.2 kb ompH-amplification usingEcoRI, HindIII and CfoI endonucleases produced 7 different patterns for the twenty five isolates of the fourP.multocida serotypes. The PCR-RFLP of the ompH gene was found to be potentially a useful method fortyping of P. multocida and therefore, for studying the epidemiology of P. multocida infections.